Molecular Mechanisms of Intracellular Trafficking

The precise regulation of secretory and endocytic trafficking is inevitable to ensure cellular homeostasis and to prevent a disease. In order to identify novel regulators, we have developed a number of cell assays and techniques to facilitate functional genetic screens in collaboration with the group of Holger Erfle at BioQuant. Numerous novel trafficking regulators, including ncRNAs, were identified. Based on this data pool, we decided to proceed in two directions with projects in both basic and applied research. Firstly, we continue to analyze endocytosis of integrins, which are the major receptors for the interaction of cells with the extracellular matrix. We aim to understand how information about cell shape, differentiation status, and adhesive properties of the surrounding defines integrin trafficking routes. Secondly, in the applied research project, we investigate how the functional and morphological features of the endocytic machinery influence cargo internalization and release.